Garvieacin Q, a novel class II bacteriocin from Lactococcus garvieae BCC 43578.

نویسندگان

  • Amonlaya Tosukhowong
  • Takeshi Zendo
  • Wonnop Visessanguan
  • Sittiruk Roytrakul
  • Laphaslada Pumpuang
  • Janthima Jaresitthikunchai
  • Kenji Sonomoto
چکیده

Lactococcus garvieae BCC 43578 produces a novel class II bacteriocin, garvieacin Q (GarQ), 70 amino acids in length and containing a 20-amino-acid N-terminal leader peptide. It is cleaved at the Gly-Gly site to generate the mature GarQ (5,339 Da), which is especially inhibitory against Listeria monocytogenes ATCC 19115 and other L. garvieae strains.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Genome sequence of Lactococcus garvieae IPLA 31405, a bacteriocin-producing, tetracycline-resistant strain isolated from a raw-milk cheese.

This work describes the draft genome sequence of Lactococcus garvieae IPLA 31405, isolated from a traditional Spanish cheese. The genome contains a lactose-galactose operon, a bacteriocin locus, two integrated phages, a transposon harboring an active tet(M) gene, and two theta-type plasmid replicons. Genes encoding virulence factors were not recorded.

متن کامل

Genome sequence of the bacteriocin-producing strain Lactococcus garvieae DCC43.

This work describes the draft genome sequence of Lactococcus garvieae DCC43. The 2.2-Mb draft genome contains 2,227 predicted protein-coding genes, among which is a region encoding the bacteriocin garvicin ML. No antibiotic resistance genes or capsule-related virulence genes were identified. Two plasmid replication regions indicate that this strain likely contains plasmids. Comparative genomics...

متن کامل

Complete genome sequence and description of Lactococcus garvieae M14 isolated from Algerian fermented milk

We describe using a polyphasic approach that combines proteomic by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) analysis, genomic data and phenotypic characterization the features of Lactococcus garvieae strain M14 newly isolated from the fermented milk (known as raib) of an Algerian cow. The 2 188 835 bp containing genome sequence displays a metaboli...

متن کامل

Identification and Characterization of Lactococcus Garvieae and Antimicrobial Activity of Its Bacteriocin Isolated from Cow’s Milk

This study was conducted to isolate lactic acid bacteria (LAB) from cow’s milk and characterize through morphological, physiological, biochemical, carbohydrate fermentation and its antimicrobial property. The bacteria were isolated by serial dilution of milk samples and confirmed through biochemical, physiological and 16s rRNA sequencing. The antibiotic susceptibility test of bacteria, antimicr...

متن کامل

Functional genetic analysis of the GarML gene cluster in Lactococcus garvieae DCC43 gives new insights into circular bacteriocin biosynthesis.

Garvicin ML (GarML) is a circular bacteriocin produced by Lactococcus garvieae DCC43. The recently published draft genome of this strain allowed determination of the genetic background for bacteriocin production. Bioinformatic analysis identified a gene cluster consisting of nine open reading frames likely involved in the production of and immunity to GarML. The garA gene encodes the bacterioci...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Applied and environmental microbiology

دوره 78 5  شماره 

صفحات  -

تاریخ انتشار 2012